Cytokinin dehydrogenase activity
WebApr 9, 2024 · N 6-(2-Isopentenyl)adenine (2iP) and zeatin are natural N 6-substituted purine cytokinins that are used for in vitro culture protocols, mainly to promote axillary shoot proliferation and adventitious shoot induction.One of the mechanisms that regulates local cytokinin homeostasis in the in vitro plant is their degradation by cytokinin … WebJun 1, 2024 · The overexpression of CycD3 is sufficient to induce cytokinin-independent shoot formation in the calli 98, and the loss of CycD3 activity reduces the ability of exogenous cytokinins to induce ...
Cytokinin dehydrogenase activity
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WebSep 28, 2007 · The biological activity of cytokinin nucleotides is still unclear, since they usually show no activity in bioassays but can bind to certain cytokinin receptors … WebIntroductionZinc (Zn) as an essential micronutrient and cytokinin as phytohormone not only regulate plant growth but also play fundamental roles in plant tolerance against drought stress. Understating the function and the role of cytokinin in
WebWendy A. Stirk mainly investigates Botany, Cytokinin, Axenic, Chlorophyceae and Zeatin. Wendy A. Stirk has included themes like Micropropagation and Tissue culture in her Botany study. ... antioxidant and antibacterial activity of Hypoxis hemerocallidea Fisch. & C.A. Mey (28 citations) ... Her work deals with themes such as Malate dehydrogenase ... WebSep 16, 2015 · Cytokinin dehydrogenase activity was measured using a modified end-point method described earlier . The reaction mixture (total volume of 0.6 ml in 1.5 ml tube) consisted of 100 mM McIlvaine buffer, pH 5.0 or 7.5, electron acceptor, substrate (dissolved in dimethyl sulfoxide that reached the final concentration of 2.5% in the reaction mixture ...
WebJun 1, 2024 · Cytokinins play two roles in this process: (1) promoting the growth of meristems to provide a source of stem cells as a prerequisite for leaf initiation and … WebSep 1, 2007 · Variable distribution of cytokinin oxidase/dehydrogenase activity (CKX: EC 1.5.99.12) in leaves of young pea seedlings (cv. Manuela) during the period needed for full leaf bud expansion was observed.
WebMar 4, 2014 · We have developed a N 6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins.Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface of a Pt …
WebJul 1, 2002 · Spectrophotometric methods for determining the activity of cytokinin oxidase/cytokinin dehydrogenase (EC 1.5.99.12) were developed and optimized. A sensitive end-point method based on a combination of the electron acceptor 2,6-dichlorophenolindophenol and Schiff base formation of the reaction product with 4 … darkclaw lobster tbc classicWebModifying the cytokinin content in plants is a means of improving plant productivity. Here, we report the development and biological activity of compound TD-K (1-(furan-2 … darkclaw lobster tbcWebNov 29, 2024 · Cytokinins have opposing actions in shoot and root growth—actions shown to involve cytokinin dehydrogenase (CKX), … darkclaw lobster wowheadWebThe cytokinin zeatin is named after the genus of corn, Zea. Cytokinins ( CK) are a class of plant hormones that promote cell division, or cytokinesis, in plant roots and shoots. They … dark claw orsWebMay 15, 2012 · Cytokinin dehydrogenase (CKX) catalyzes the irreversible degradation of cytokinins (CKs). CKs play a role in the regulation of hydrogen peroxide (H 2 O 2) accumulation while H 2 O 2 is involved in chlorophyll degradation. Here, we elucidated how CKX differentially regulates representative CK levels and indirectly influence H 2 O 2 … bisexual trendsWebEven though, it has already been established since 1980s that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; the potential applications of manipulating these enzymes have mostly been speculated to have a high potential in the biotechnology industry and spreads to agriculture, horticulture and agroforestry ... bisexual typesWebThe cytokinin dehydrogenases (CKX; EC 1.5.99.12) are a protein family that maintains the endogenous levels of cytokinins in plants by catalyzing their oxidative degradation. The CKX family in maize (Zea mays L.) has thirteen members, only two of which - ZmCKX1 and ZmCKX10 - have previously been characterized in detail. darkclaw marvel dc