WebDec 24, 2008 · No differences in the growth rates of TSP2-null and WT wound fibroblasts were observed. Wound fibroblasts (1 × 10 5 cells/ml) were mixed in a 1-mg/ml collagen (PureCol; Inamed Biomaterials, Fremont, CA) preparation (kept at 4C) and plated in 24-well dishes precoated with 1% agarose, and incubated at 37C/5% CO 2. Three hr later, the …
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WebThe DRG neurons were acutely dissociated as described. 22 25 Dispersed neurons were suspended in F12 medium (Sigma-Aldrich; St. Louis, Missouri, USA) (pH 7.2–7.3, 10% FCS, plated on laminin/poly-d-lysine coated (60 μL/mL) (Inamed Biomaterials; Fremont, California, USA) cover slips and incubated at 95% O 2 and 5% CO 2. Two hours after plating ... WebMar 1, 2011 · Acidified bovine type I collagen solution (3.1 mg ml −1 PureCol; Inamed Biomaterials, CA) was mixed with 10× minimum essential medium and neutralized with 0.8 M NaHCO 3 to prepare modules as described elsewhere [6] using 0.76 mm ID polyethylene tubing (Intramedic PE60, Benton Dickson).
WebNov 30, 2007 · Preparation of Bioglass-Loaded Sponges Known masses of BG were sterilized by suspending the material in 95% ethanol in microcentrifuge tubes for 30 min, followed by 2 washes in sterile PBS. Biopsy punches (Acuderm, Ft Lauderdale, FL, USA) were used to create disks (5 × 2.5 mm) from collagen sponges under sterile conditions. WebFeb 19, 2014 · Biomaterials 32:2775–2785, 2011. Article Google Scholar Miron-Mendoza, M., J. Seemann, and F. Grinnell. Collagen fibril flow and tissue translocation coupled to …
WebType I collagen was from Inamed BioMaterials (Fremont, CA, USA). EDTA-free protease inhibitor cocktail tablets were from Roche Pharma (Basel, Switzerland). Phalloidin red (actin-stainTM 555 Phalloidin) was from Cytoskeleton, Inc (Denver, CO, USA). Antibodies used were as follows: mouse anti-FLAG M2 (Sigma-Aldrich, Taufkirchen, WebJan 7, 2024 · Approximately 400 MDCK cells were suspended in 0.4 mL of ice-cold MEM (Sigma-Aldrich, St. Louis, MO, USA) containing 2.9 mg/mL collagen (PureCol; Inamed Biomaterials, Fremont, CA, USA), 10 mM ...
WebJul 24, 2014 · Cells were maintained in serum-free LHC-9 medium in collagen-coated (PureColTM, Inamed Biomaterials) flasks. The human alveolar type-II-like carcinoma cell line A549 and the human leukemia monocyte cell line THP-1 were obtained from the American Type Culture Collection (Manhasset, VA, USA).
WebInamed was founded in 1998 as a spin-off of the GSF National Research Center for Environment and Health (now called Helmholtz Research Centre). Therefore INAMED … fly away home vhs ebayWebApr 1, 2010 · To improve healing of site-specific bone defects, a promising approach consists in the implantation of biomaterials loaded with MSCs. It was demonstrated that … fly away home the movieWebJun 1, 2009 · of collagen type I (Inamed Biomaterials, Fremont, CA) in phosphate buffered saline at 37°C. for 16 h during which primary amines react with the N-hydroxysuccinimide … fly away home vhs 1996WebJul 19, 2016 · VEGFR2 contains various tyrosine residues within its intracellular domain that become phosphorylated upon VEGF stimulation, including Tyr 949, Tyr 1052, Tyr 1057, Tyr 1173, and Tyr 1212 (corresponding to the numbering in the mouse VEGFR2). The interaction partners and in vivo role of these individual phosphorylation sites are now being unraveled. greenhouse contractors nashvilleWebInamed Biomaterials 800-883-8220. Title: Microsoft Word - A4a Astrocyte Cell Culture.docx Author: ahuang18 Created Date: 11/3/2008 10:11:21 AM ... greenhouse controlWebJun 16, 2009 · Collagen is a convenient biomaterial for biomechanical studies for a number of reasons: a) ... (Invitrogen), bovine collagen diluted to desired concentration (from 3.1 mg/mL or 6.4 mg/mL batch, Inamed Biomaterials, Fremont, CA), a few µL of 1 M sodium hydroxide (NaOH, Sigma, St. Louis, MO) ... greenhouse contractors in floridaWebINAMED®Silicone-Filled Breast Implants are constructed with barrier shell technology resulting in a low diffusion silicone elastomer shell and are filled with a soft, cohesive silicone gel. All... greenhouse containers rectangular nusery pots