WebbSPR prism temperature: 25 °C. Steps: Weigh 40 mg EDC and 10 mg NHS into a suitable vial and dilute with 0.9 mL of water. Dissolve and inject as quickly as possible after diluting. Inject over ... WebbNicoya Lifesciences Inc. show all products Specifications. Performance - Association Rate (kon) 1×10 3 –1 ... OpenSPR Amine Coupling Kit - Small Molecules 260.00 CHF. OpenSPR Amine Coupling Kit - Small Molecules Art No HBS-EP. Running Buffer HBS-EP pH 7.4 240.00 CHF.

Amine coupling of ligand to Biacore sensor chips - Cytiva

WebbAmine Coupling Kit is designed to be used with carboxylated sensor surfaces from Cytiva . Ligands are coupled via primary amine groups to the sensor surface for subsequent analysis. Reagent preparation WebbN-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS) is often included in EDC coupling protocols to improve efficiency or create dry-stable (amine-reactive) … check att texts online

Site-Specific Photoaffinity Bioconjugation for the Creation of

Webbvia its carboxyl groups. To perform the coupling, the carboxyl groups on the ligand can be activated with standard EDC/NHS chemistry to chemically couple the ligand to the sensor chip (Figure 1). Nicoya’s Amine Coupling Kit contains all reagents necessary to perform this coupling and has been optimized for best performance. WebbN-hydroxysuccinimide (NHS) or its water-soluble analog (Sulfo-NHS) is often included in EDC coupling protocols to improve efficiency or create dry-stable (amine-reactive) intermediates. EDC couples NHS to carboxyls, forming an NHS ester that is considerably more stable than the O -acylisourea intermediate while allowing for efficient conjugation … WebbImageQuant™ TL software, Sensor Chip CM5, Amine Coupling Kit, Surfactant P20, HBS-EP+ buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05% Surfactant P20), 50 mM NaOH, Cy™3 ... were immobilized using standard amine coupling to a response of 7 000 RU. The samples were diluted in HBS-EP+ with 1% BSA and injected over the surface … check attribute python