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Peasy庐-blunt zero cloning kit

WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为:95 ℃预变性2 min;95 ℃ 30 s,55 ℃ 30 s,72 ℃ 1 min,30个循环;72 ℃ 延伸 2 min。PCR产物进行胶回收后,连接至pEASY-Blunt Zero Cloning Kit克隆载体,转化大肠杆菌感受态DH5α中,挑取单克隆测序。

pEASY-Blunt Zero Cloning Kit - Alibaba.com

WebpEASY® -Blunt Cloning Kit Catalog Number:CB101-01 Price:$89.02 Specification: 20 rxns 60 rxns Quantity: Manual Vector Information MSDS COA Inquire Now Buy Now Add … WebpEASYpEASY ?-T1 Cloning Kit 目录号:...测序用 M13 F,M13 R,T7 promoter 引物测序,进行 序列 分析。 注意! 1. ... pGM- T载体全序列 pGM-T载体全序列pGM- T 载体序列 pGM- T 载体 是在60碱基处用EcoRV切开 (星号处),然后在3’端加T形成的 (加入的T未包含在此... pEASY T 载体 pEASY T载体_基础医学_医药卫生_专业资料。加强零背景载体的监督, 分子生物学家... thor hd 640 review https://vip-moebel.com

pEASY CloNiNg VECtor - Biologica

Web平末端载体pEASY-blunt ZERO Cloning Kit和感受态细胞Trans1-T1Chemically Competent Cell(购自全式金公司)。互补载体pCAMBIA 1301,过表达载体pCAMBIA 130135SN。 1.2 方法. 1.2.1 拟南芥植株培养 WebThe Zero Blunt™ PCR Cloning Kit is offered in a variety of configurations: with One Shot™ TOPO10 Chemically Competent E. coli (K2700-20 and K2700-40) and without competent … WebZero™ Blunt™ TOPO™ kits are available with a variety of competent cells, or without competent cells, depending on your needs and budget. Features of the Zero™ Blunt™ TOPO™ PCR Cloning Kits: Fast and easy— go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time. Efficient— obtain up to 95% clones with ... umaine flagship match 2020

PEASY-T1载体全序列_文档下载

Category:棉花GhPHOT1和GhPHOT2基因的克隆及功能鉴定.pdf - 原创力文档

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Peasy庐-blunt zero cloning kit

pEASY-Blunt Zero Cloning Kit - Alibaba.com

WebProduct Details pEASY® -T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. •5 minutes fast ligation of Taq-amplified PCR products. •High cloning efficiency. Web一、 采购人名称: 石河子大学 二、 供应商名称: 石河子市北泉镇诺泰实验用品经销部 三、 采购项目名称: 石河子大学网上超市项目 四、 采购项目编号: ***** 五、 合同编号: 11n ***** 六、 合同内容:

Peasy庐-blunt zero cloning kit

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WebThe Zero Blunt™ TOPO™ PCR Cloning Kit for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages depending upon your needs: General cloning: TOP10 cells (Cat. No. K2800-20, K2800-40) WebpEASY® Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed …

WebHiPure Circulating DNA Kit IVD3182 Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol HiPure Blood DNA Mini Kit D3111 Isolation total DNA from 200μl whole blood News View more Magen helps the success of Cell-free DNA Research and Application Magen helps the success of Cell-free DNA Research and …

WebZero Blunt ® TOPO ® PCR Cloning Kits are shipped on dry ice. Kits supplied with competent cells containa box with Zero Blunt ® TOPO ® PCR Cloning reagents (Box 1) and a box with One Shot ® E. coli (Box 2). Zero Blunt ® TOPO ® PCR Cloning Kit supplied with the PureLink ® Quick Plasmid Miniprep Kit (Cat. no.K2800-02) is shipped with an ... WebpEASY-Blunt Zero Cloning Kit The pEASY -Blunt Zero Cloning Vector contains a suicide gene. The insertion of blunt-end PCR products using advanced topoisomerase technology (Topo cloning) disrupts the …

WebOct 1, 2016 · Features of the pEASY-Blunt Zero Cloning Kit 5 minutes fast insertion of blunt-end PCR products.by topoisomerase. High cloning …

WebpEASY® -Blunt Zero Cloning Kit Catalog Number:CB501-01 Price:$112.00 Specification: 20 rxns 60 rxns Quantity: Manual Vector Information MSDS COA Inquire … umaine fogler library databaseWebThe “pEASY ” cloning vectors provide a highly efficient, 5 minutes, ... Cloning kits are supplied with free Trans1-T1 Phage Chemically Comptent Cell. Publications with pEASY vector Applications: > Sequencing; > ... pEASY®-Blunt Zero p (3956 bp) U C o ri l a c Z A mp ic l n K a n a m y c i n umaine financial aid scholarship universeWeb本发明公开了一种拟南芥长链非编码RNA‑DANA1及其在植物干旱耐受性的应用,涉及植物的基因工程领域。其中,所述的DANA1核苷酸序列如SEQ ID NO.1所示。本发明通过克隆拟南芥长链非编码RNA‑DANA1的全长转录本,并构建过表达载体获得DANA1过表达转基因植株,过表达DANA1后植物表现出失水速率减慢,对 ... thor hdw2401ss specsWebpEASY-Blunt (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer umaine fogler libraryWebThis PCR Cloning Kit contains an optimized Cloning Mix containing a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background. umaine fogler library hoursWebpEASY®-Blunt Simple TOPO Cloning Kit. p EASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of the pEASY® -Blunt Cloning Vector. It is designed for cloning and … thor hd movie downloadWebpEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but no … u maine grocery store